Effect of Resveratrol on H2O2-induced Oxidative Damage of SH-SY5Y Cells
Objective To study the effect of resveratrol(Res)on H2O2-induced oxidative damage of human neuroblastoma SH-SY5Y cells.Methods The oxidative damage model of SH-SY5Y cells induced by a certain dose of H2O2 was established.The effects of Res on the viability of SH-SY5Y cells after oxidative damage were investigated and the subsequent experimental doses were screened.SH-SY5Y cells were treated with 20,10,5 and 1 μmol/L Res for 24 h,then 1.2 mmol/L H2O2 was added.After 24 h,cell viability was measured by MTT and growth curve method,cell cycle was measured by flow cytometry,and cell morphology was observed by HE staining.Superoxide dismutase(SOD)activity,glutathione peroxidase(GSH-Px)activity,malondialdehyde(MDA)content and extracellular lactate dehydrogenase(LDH)activity were determined by detection kits.Results 1.2 mmol/L H2O2 caused oxidative damage to SH-SY5Y cells,decreased cell viability,caused change of cell cycle and cell morphology,decreased SOD and GSH-Px activity,and increased MDA content and extracellular LDH activity(P<0.01).Compared with model group,the cell viability and the cell percentage of G0/G1 and G2/M phase of 20,10 and 5 μmol/L Res treated groups increased,and the percentage of S phase cells decreased significantly(P<0.01);meanwhile,the intracellular SOD and GSH-Px activities increased(P<0.01),and MDA content and extracellular LDH activity decreased significantly(P<0.01).Conclusion 20,10 and 5 μmol/L Res can protect SH-SY5Y cells from oxidative damage induced by H2O2.
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