Objective To explore the rapid identification technology of Hedyotis hedyotidea(DC.)Merr.by infrared spectroscopy(IR).Methods The overall analysis of the medicinal materials of Hedyotis hedyotidea(DC.)Merr.was performed by FT-IR.Results The characteristic absorption peaks of IR fingerprint of Hedyotis hedyotidea(DC.)Merr.were obtained as follows:3418,2925,2854,1723,1650,1514,1401,1319,1267,1155,1104,1054,1030,919,811,782,763,664,592,538,520 cm-1.1267,1030 cm-1 and 919 cm-1 were the IR characteristic indicative peaks of ursolic acid,which was one of the analgesic and anti-inflammatory components of Hedyotis hedyotidea(DC.)Merr..The IR similarity among the samples was in the range of 0.9003-0.9895,thus the IR similarity threshold of Hedyotis hedyotidea(DC.)Merr.was proposed as 0.90.Conclusion The FT-IR fingerprint of Hedyotis hedyotidea(DC.)Merr.can provide scientific basis for its rapid identification and quality control.