Objective To isolate and purify an oligosaccharide monomer from Alhagi honey and elucidate its molecular structure.Methods Various chromatographic techniques were employed to extract and purify Alhagi honey extract.The molecular structure of the isolated Alhagi honey oligosaccharide was determined using high-performance liquid chromatography-electrospray ionization mass spectrometry(HPLC/ESI-MS),infrared spectroscopy(IR),and nuclear magnetic resonance spectroscopy(NMR).Results After separation and purification,one oligosaccharide monomer with relative molecular weight of 504 and structure of α-D-Glc-(1→6)-β-D-Glc-(1-6)-D-Glc was obtained,which was confirmed to be isomaltooligosaccharide.Conclusion In this study,isomaltooligosaccharide is isolated and purified from Alhagi honey for the first time.
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