Comparison of Molecular Weight Distribution and In Vitro Antioxidant Capacity of Small Molecule Peptides Prepared by Two Methods from Cordyceps Militaris
Objective To investigate the molecular weight distribution of small molecular peptides from Cordyceps militaris prepared by compound enzymatic hydrolysis and enzyme-high pressure steaming coupled hydrolysis and the difference of antioxidant activity in vitro.Methods The molecular weight distribution of peptides obtained by two methods was detected by gel chromatography,and the total antioxidant capacity(T-AOC),hydroxyl radical and super-oxide anion radical scavenging capacity were measured respectively to evaluate the in vitro antioxidant capacity of the two groups of peptides.Results The molecular weight distribution of peptide samples prepared by two methods was similar.The proportion of components with molecular weight less than 10×103 Da was near 60%,and the proportion of components with molecular weight less than 5×103 Da was about 31%.The active peptides with molecular weight less than 10×103 Da and molecular weight less than 5×103 Da prepared by two methods showed better hydroxyl radical and superoxide anion radical scavenging ability than reducing glutathione(GSH).The T-AOC activity of active pep-tides with molecular weight less than 5×103 Da obtained by compound enzymatic hydrolysis was significantly higher than that of GSH.Conclusion The molecular weight distribution of peptides prepared by the two methods showed no significant difference,and both had good in vitro antioxidant activity.
万方数据
维普
