GC-MS Analysis and Comparative Study of Volatile Components in Pomelo Flowers Using Three Extraction Methods
Objective To investigate the effects of different pretreatment methods on the types and contents of volatile organic compounds(VOC)in pomelo flowers.Methods Taking pomelo flowers as experimental research objects,the samples were pretreated by three different extraction methods:static headspace(SHS),solid-phase microextraction(SPME)and purge and trap(PT).The VOCs in pomelo flowers were qualitatively and relative quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS),and principal component analysis on the volatile components was performed.Results Using three different extraction methods combined with GC-MS,a total of 70 compounds belonging to five categories,namely terpenes,alcohols,esters,amines,and alkanes,were identified in pomelo flowers.Among them,the SPME method detected the highest number of compounds,including 44 compounds belonging to five categories.The SHS and PT methods detected 9 compounds in four categories and 30 compounds in five categories,respectively.Principal component analysis showed that α-pinene,γ-terpinene,α-phellandrene,and lemonene were the main contributing substances of the volatile components in pomelo flowers.Conclusion The volatile components of pomelo flowers detected by different extraction methods combined with GC-MS technology are different.This study can provide experimental basis and data support for the medicinal research and development of pomelo flower resources.
static headspace(SHS)solid-phase microextraction(SPME)purge and trap(PT)gas chromatography-mass spectrometry(GC-MS)pomelo flowervolatile organic compound
万方数据
维普
