Study on Optimization of Deep Eutectic Solvents Extraction Process by Response Surface Method and Antioxidant Activity of Total Flavonoids from Flower of Tilia Amurensis Rupr
Objective To investigate the effect of different types of deep eutectic solvents on the extraction rate of total flavonoids from the flower of Tilia amurensis Rupr.,optimize the process and study the antioxidant activity of its total flavonoids.Methods Based on a single factor experiment,a quadratic regression model was established using the response surface design method to select the factors that showed significant impact on the extraction rate of total flavonoids from the flower of Tilia amurensis Rupr.,using ultrasound power,ultrasound time and solvent water content as independent variables and the extraction rate of total flavonoids as dependent variable.The in vitro antioxidant capacity of the total flavonoids from the flower of Tilia amurensis Rupr.was judged based on its scavenging effects on ABTS and DPPH free radicals.Results The optimal conditions for the extraction of total flavonoids from the flower of Tilia amurensis Rupr.were as follows:mixture of choline chloride and lactic acid(molar ratio 1:2)was used for extraction,the water content was 50%,the extraction time was 30 min,and the ultrasonic power was 200 W.Under these conditions,the highest extraction rate(10.61%)was obtained.The total flavonoids from the flower of Tilia amurensis Rupr.showed good antioxidant capacity with scavenging rates of 93.27%and 90.85%for ABTS and DPPH free radicals,respectively,and IC50 values of 0.021 and 0.0045 mg/ml,respectively.Conclusion The extraction process of total flavonoids from the flower of Tilia amurensis Rupr.is optimized by response surface methodology,and a simulated regression equation is established,which can be used to optimize the extraction process parameters.The results of antioxidant experiments show that the total flavonoids have good antioxidant activities in vitro and can be used to develop new antioxidant drugs.
deep eutectic solventflower of Tilia amurensis Rupr.process optimizationtotal flavonoidsantioxidant activity
万方数据
维普
