Rapid Determination of Chloramphenicol,Thiamphenicol and Florfenicol Residues in Mutton by QuEChERS-UPLC-MS/MS
Objective To establish an analytical method for the determination of chloramphenicol,thiamphenicol and florfenicol residues in mutton by QuEChERS-ultra performance liquid chromatography-tandem triple quadrupole mass spectrometry(QuEChERS-UPLC-MS/MS).Methods The samples were homogenized by a grinder and extracted with acetonitrile.The extract was purified by QuEChERS-1010 and QuEChERS-0101,determined by UPLC-MS/MS and quantified by internal standard method.For detection,electrospray negative ion scanning and multiple reaction monitoring were adopted.Results Chloramphenicol,thiamphenicol and florfenicol showed good linear relationships in the range of 4-50 μg/L,with correlation coefficients above 0.998.At the concentrations of 5.0,10.0,30.0 μg/kg,the recoveries of three drugs were in the range of 93.17%-109.91%,and the relative standard deviations(RSDs)ranged from 2.33%to 6.14%.The limits of detection of the method were 0.01-0.03 μg/kg,and the limits of quantification were 0.02-0.09 μg/kg.Conclusion This method is simple,rapid,accurate and sensitive,and meets the detection requirements for the determination of chloramphenicol,thiamphenicol and florfenicol residues in mutton.
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