Preliminary Study on Distribution of Walnut Peptides with Different Molecular Weights in Mice
Objective To prepare walnut peptides with different molecular weights and preliminarily investigate the distribution of walnut peptides in mice after being labeled with Cy7.Methods Walnut peptides were prepared by degradation of walnut dregs with different enzymes,the relative molecular weight(Mr)of walnut peptides was determined by GPC chromatography,and the Cy7-labeled walnut peptides were used for in vivo imaging of mice to observe their distribution in mice.Results The Mr of samples obtained by different enzymes was different.Sample Ⅰ had a high proportion of small molecular weight components and a high yield.Different samples were labeled by Cy7 to show different fluorescence intensities in mice.Cy7-sample Ⅰ showed fluorescence signals in the brain,and Cy7-sample Ⅱ and Cy7-sample Ⅲ only showed strong fluorescence signals in the tail of the injection.Conclusion Walnut peptides prepared by different enzymes have different distributions in mice,and sample Ⅰ may contain peptides which enhance brain fluorescence.
万方数据
维普
