Objective To establish a method for the determination of paeoniflorin in Taohong Siwu Decoction by LC-MS/MS.Methods The separation was achieved on an Agilent Eclipse Plus C18(5 μm,2.1 mm×50 mm)column using a gradient mobile phase system of methanol-water(containing 0.1%formic acid).ESI negative ionization and multiple reaction monitoring mode(MRM)were used for mass spectrum acquisition.Results The linear range of paeoniflorin was 0.1 to 50 μg/ml.Specificity,recovery,precision and uniformity all met the requirements.Conclusion This method has strong specificity,high sensitivity,short analysis time,and can be used for the determination of paeoniflorin in Taohong Siwu Decoction.
维普
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