Simultaneous Determination of Ten Mycotoxins in Vespae Nidus and Its Preparations by QuEChERS EMR-Lipid Combined with Isotope Dilution-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
Objective To establish a method for simultaneous determination of ten mycotoxins in Vespae Nidus and its preparations by QuEChERS EMR-Lipid combined with isotope dilution-ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods The samples were extracted with 80%acetonitrile solution and purified by QuEChERS EMR-Lipid.Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used for determination in mode of multiple reaction monitoring(MRM),and internal standard method was used for quantification.Results The 10 mycotoxins showed good linearity within their respective concentration range(r>0.999).The average recoveries were 83.8%-107.1%(RSDs<6.5%)at low,medium,and high spiked levels.The LOQs were in the range of 0.18-129 μg/kg.Conclusion The method has simple pretreatment steps,good purification effect,and improved sample detection efficiency,and the internal standard method is accurate and reliable.It is suitable for simultaneous detection of 10 mycotoxins in Vespae Nidus and their preparations.
QuEChERS EMR-LipidVespae Nidusmycotoxinultra performance liquid chromatograph-tandem mass spectrometry(UPLC-MS/MS)isotope internal standard
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