Objective To establish a method for the simultaneous determination of biotin in special dietary foods by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Methods The samples were added internal standard substance and warm water of 40℃,and were ultrasonically extracted after oscillation and mixing,then cooled to room temperature.The pH values of the samples were then adjusted to 1.6±0.2 with perchloric acid.After centrifugation,the pH values of the supernatant were adjusted to 4.5±0.2 by sodium hydroxide.After being centrifuged again,the supernatant was filtered by 0.22 μm filter membrane,then was separated with Kinetex RR-C18(100 mm×2.1 mm,2.6 μm)column,using 0.1%formic acid aqueous solution-acetonitrile as mobile phase.The quantitative analysis was performed through internal standard method.Results The biotin had good linear relationship in the range of 1-50 ng/ml(r>0.999).The limit of detection was 0.003 μg/g,and the limit of quantitation was 0.01 μg/g.The content of biotin in sample extract solution was stable within 10 h,and the RSD was 5.87%.The average recoveries at the spiked levels of 5,15,30 ng/ml were 90.7%-116.9%,with RSDs of 1.71%-4.42%(n=6).Conclusion This method has fast detection speed,high sensitivity and good repeatability,and is suitable for rapid detection and confirmatory analysis of biotin in special dietary foods.
biotinspecial dietary foodultra performance liquid chromatography-tandem mass spectrometry
万方数据
维普
