Determination of Residual Ethephon in Baise Mango by Gas Chromatography-Tandem Mass Spectrometry
Objective To establish a method for determination of ethephon residues in mango from Baise by gas chromatography-tandem mass spectrometry.Methods The factors including extraction reagent,pH value,derivative agent,derivative agent dosage and derivatization time were optimized.Under the optimal conditions,ethephon residues in mango were determined by triple-quadrupole gas chromatography-tandem mass spectrometry,and the reliability of the method was evaluated.Results The optimal extraction conditions were as follows:methanol was used as extraction reagent,and the pH value was adjusted to 3,and the optimal derivatization conditions were as follows:trimethylsilyl diazomethane was used as derivative agent with dosage of 0.8 ml,and the reaction time was 5 min.The linear equation was Y=317 481.36X+9763.62,r=0.9998.The detection limit was 0.003 mg/kg,the quantitative limit was 0.009 mg/kg,and the average recovery was 90%-99%.Ethephon residues were detected in only 5 mango samples from 13 regions of Baise,and the residues were all less than 0.01 mg/kg.Conclusion The results of stability,repeatability,recovery and precision test and mango sample determination show that the method is accurate and easy to operate,and can be used in the quantitative analysis on ethephon residues in mango.
gas chromatography-tandem mass spectrometrymangoethephonoptimization of extraction
万方数据
维普
