Objective To establish a method for simultaneous determination of vitamin A(VA)and vitamin E(VE)in milk and dairy products by high performance liquid chromatography with fluorescence detector(HPLC-FLD).Methods The samples were hydrolyzed and saponified,liquid-liquid extracted,evaporated and concentrated,then were separated on a PFP column,using methanol-water(9:1,V/V)as mobile phase of isocratic elution,and were detected by HPLC-FLD.External standard method was adopted for quantification.Results The standard curves of VA and 4 kinds of VE all exhibited ideal linear relation over the concentration range of 0.10-5.00 μg/ml,the limit of detection was 0.0100 μg/g,and the limit of quantification was 0.0400 μg/g.The average recoveries of VA and 4 kinds of VE were 85.0%-97.0%with RSDs＜6.41%(n=6)and 84.0%-101%with RSDs＜7.04%(n=6),respectively.Conclusion The method is simple,rapid,sensitive and accurate,which can be used for simultaneous determination of VA and VE in milk and dairy products.
关键词
高效液相色谱-荧光检测器法/维生素A/维生素E/乳及乳制品
Key words
high performance liquid chromatography with fluorescence detector/vitamin A/vitamin E/milk and dairy products
维普
万方数据