Simultaneous Determination of Vitamin A and Vitamin E in Milk and Dairy Products by High Performance Liquid Chromatography with Fluorescence Detector
Objective To establish a method for simultaneous determination of vitamin A(VA)and vitamin E(VE)in milk and dairy products by high performance liquid chromatography with fluorescence detector(HPLC-FLD).Methods The samples were hydrolyzed and saponified,liquid-liquid extracted,evaporated and concentrated,then were separated on a PFP column,using methanol-water(9:1,V/V)as mobile phase of isocratic elution,and were detected by HPLC-FLD.External standard method was adopted for quantification.Results The standard curves of VA and 4 kinds of VE all exhibited ideal linear relation over the concentration range of 0.10-5.00 μg/ml,the limit of detection was 0.0100 μg/g,and the limit of quantification was 0.0400 μg/g.The average recoveries of VA and 4 kinds of VE were 85.0%-97.0%with RSDs＜6.41%(n=6)and 84.0%-101%with RSDs＜7.04%(n=6),respectively.Conclusion The method is simple,rapid,sensitive and accurate,which can be used for simultaneous determination of VA and VE in milk and dairy products.
high performance liquid chromatography with fluorescence detectorvitamin Avitamin Emilk and dairy products
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