Simultaneous Determination of Eight Components in Wuling Capsules by QAMS
Objective To establish a method for simultaneous determination of saikosaponin a,saikosaponin d,schisandrin A,schisandrin B,schisandrol A,tanshinone ⅡA,salvianolic acid B and cryptotanshinone in Wuling Capsules by quantitative analysis of multi-components by single marker(QAMS).Methods The HPLC separation was achieved on an Agilent ZORBAX SB-C18 column(4.6 mm×250 mm,5 μm)with acetonitrile-0.1％phosphoric acid as mobile phase of gradient elution,which was at a flow rate of 1.0 ml/min.The determination wavelength was 210 nm.The column temperature was set at 30℃.With schisandrol A as the internal reference,the relative correction factors of saikosaponin a,saikosaponin d,schisandrin A,schisandrin B,tanshinone ⅡA,salvianolic acid B and cryptotanshinone were calculated.The contents of 8 components were calculated and compared with those determined by the external standard method(ESM).Results Saikosaponin a,saikosaponin d,schisandrin A,schisandrin B,schisandrol A,tanshinone ⅡA,salvianolic acid B and cryptotanshinone showed good linear relationships within the ranges of 9.58-191.50,9.10-182.02,1.04-25.89,2.00-50.10,5.37-134.35,1.00-25.08,8.27-206.72,1.07-26.67 µg/ml(r≥0.9992).RSDs of precision,repeatability and stability tests were all less than 2％.The average recoveries were 97.06％-99.95％,with the RSDs of 0.53％-1.73％.The results of QAMS were consistent with those of ESM.Conclusion The method is simple and accurate,and can be used to evaluate the quality of Wuling Capsules.
万方数据
维普
