Simultaneous Determination of Six Index Components in Changzhong Pills by HPLC-Wavelength Switching Method
Objective To establish an HPLC method for simultaneous determination of six index components including geniposide,paeoniflorin,naringin,hesperidin,neohesperidin and berberine hydrochloeide in Changzhong Pills.Methods The chromatography was performed on Agilent ZORBAX SB-C18(4.6 mm×250 mm,5 μm)column with acetonitrile(A)-0.1%phosphoric acid(B)as mobile phases for gradient elution.The detection wavelengths were set at 238,230,284,345 nm,respectively.The flow rate was 1.0 ml/min and the column temperature was 30℃.Results The linear relationships of geniposide,paeoniflorin,naringin,hesperidin,neohesperidin and berberine hydrochloeide were good within their respective determination ranges.The correlation coefficients(r)were greater than 0.999,the average recoveries were between 97.98%-99.53%,with RSDs less than 2.0%.The contents of geniposide,paeoniflorin,naringin,hesperidin,neohesperidin and berberine hydrochloeide in three batches of Changzhong Pills were 2.307-2.395,1.806-1.895,11.392-11.959,0.507-0.544,5.314-5.433,2.406-2.497 mg/g,respectively.Conclusion The established method is simple,accurate,sensitive and reproducible,and can be used for the quality control of 6 index components in Changzhong Pills.
万方数据
维普
