Objective To simultaneously determine the contents of glycyrrhizin,tetrahydropalmatine,hesperidin and berberine hydrochloride in Shenlian Changyan Pills by HPLC-wavelength switching method.Methods The chromatographic column was Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm)column with acetonitrile-0.2%phosphoric acid solution as mobile phase for gradient elution.The flow rate was 1.0 ml/min.The detection wavelengths and switching time of glycyrrhizin,tetrahydropalmatine,hesperidin and berberine hydrochloride were 237 nm(0-7 min),283 nm(7-12 min),283 nm(7-12 min)and 345 nm(12-20 min),respectively.Results Glycyrrhizin,tetrahydropalmatine,hesperidin and berberine hydrochloride could be well separated,and the resolutions were all above 1.5.The four components had good linear relationships.The average recoveries of the four components were 100.19%,99.32%,99.64%and 100.08%,respectively,and the RSDs were 1.78%,1.69%,1.89%and 2.04%(n=6),respectively.Conclusion This method has the advantages of simple operation,accurate results,high recovery and good repeatability.It can provide some technical support for the effective quality control of Shenlian Changyan Pills.
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