Objective To establish a method for the simultaneous determination of 5 components in Yaoxitong Capsules by UPLC-MS/MS method.Methods The analysis was performed on an Endeavorsil C18(50 mm×2.1 mm,1.8 μm)column,using tonitrile-0.1%formic acid at a flow rate of 0.3 ml/min as mobile phase of gradient elution.The column temperature was 30℃,and the injection volume was 5 μl.For MS,electro-spray ion source and negative ion scanning mode,as well as multiple reaction monitoring mode were adopted.Results The primary and secondary mass spectrograms of ginsenoside Rb1,ginsenoside Rb3,ginsenoside Rd,ginsenoside Re and ginsenoside Rg1 in Yaoxitong Capsules were consistent with those of reference solution.Five constituents showed good linear relationships within their own ranges(r＞0.9931).The average recoveries were 96.7%-97.7%,with RSDs of 0.6%-1.7%.Under the optimized chromatographic conditions,the complete separation of 5 target components were obtained within 5 min.Conclusion The method is efficient,accurate and selective,and can be used for the quantitative research of five active ingredients in Yaoxitong Capsules.
关键词
腰息痛胶囊/超高液相色谱-串联质谱法/中西药复方制剂/含量测定/人参皂苷Rb1
Key words
Yaoxitong Capsules/UPLC-MS/MS/Chinese and western medicines/content determination/ginsenoside Rb1
维普
万方数据