HPLC Fingerprint and Multi-component Quantitative Analysis of Gongliuxiao Capsules
Objective To establish the HPLC fingerprint and multi-component quantitative analysis method of Gongliuxiao Capsules,and combined with chemometrics,to evaluate the quality of the drug.Methods The analysis was carried out on a Kromasil 100-5-C18(250 mm×4.6 mm,5 μm)column,using acetonitrile(A)and 0.2％phosphoric acid solution(B)as mobile phase of gradient elution(0-25 min,10％A→55％A;25-26 min,55％A→90％A;26～35 min,90％A).The flow rate was 1.0 ml/min,and the column temperature was 35 ℃.The detection wavelength was set at 310 nm,and switched to 245 nm from 15 to 35 min.Results HPLC fingerprint of Gongliuxiao Capsules was established,16 common peaks were found,and 10 common peaks were identified and quantitatively studied,including gallic acid,neochlorogenic acid,chlorogenic acid,1,2,3,4,6-pentagalloylglucose,4-coumaric acid,apigenin-7-glucoside,benzoylpaeoniforin,paeonol,furanodienone and α-cyerone.All methodological investigation results met the testing requirements.The similarity of fingerprints of 19 batches of samples were greater than 0.92.Cluster analysis grouped 19 batches of Gongliuxiao Capsules samples into 2 categories based on production years,indicating that there were certain quality differences between the samples of different years.Principal component analysis suggested that 1,2,3,4,6-pentagalloylglucose,4-coumaric acid,paeonol,furanodienone,peak 15,and α-cyerone had significant impact on the quality and could be used as index components.Conclusion The established HPLC fingerprint and multi-component quantitative analysis method can provide reference for the improvement and revision of the quality standard of Gongliuxiao Capsules.Quality evaluation only using fingerprint analysis has certain limitations and needs to be combined with chemometric methods for comprehensive evaluation.
万方数据
维普
