Determination of Multiple Bioactive Constituents of Qingfei Yihuo Tablets by NIRS and HPLC
Objective To establish a rapid method for simultaneous determination of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in Qingfei Yihuo Tablets.Methods The contents of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in Qingfei Yihuo Tablets were determined by HPLC.Partial least squares method(PLS)was used to establish the near infrared quantitative analysis model of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in the samples.Results R2 of the established near infrared quantitative analysis models for baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A were 0.9584,0.9217,0.9555,0.9512,0.9388 and 0.9221,respectively.RMSECV were 0.226,0.017,0.062,0.008,0.026 and 0.005,respectively.Conclusion The quantitative analysis models established by NIRS can be used for rapid and nondestructive determination of baicalin,chrysophanol,geniposide,mangiferin,berberine hydrochloride and praeruptorin A in Qingfei Yihuo Tablets.
万方数据
维普
