Expression of Three Nanocompartmental Shell Proteins(Encapsulin)from Streptomyces in Streptomyces Albus J1074
Objective To verify whether three types of Streptomyces nanocompartmental shell protein(encapsulin)genes could be successfully heterologouslly expressed in a type strain of Streptomyces.Methods Three encapsulin genes were recombined into the genome of Streptomyces albus J1074,and their expressions were identified by affinity chromatography,ultrafiltration,SDS-polyacrylamide gel electrophoresis(SDS-PAGE)and nano liquid chromatography tandem mass spectrometry(NanoLC-MS/MS).Results SDS-PAGE results showed that three types of recombinant S.albus J1 074 strains were all able to express differential proteins with Mr of 30×103 compared to the wild-type strain.These proteins showed high similarity with three encapsulin proteins ensured by NanoLC-MS/MS technique.Conclusion Three types of encapsulin genes were successfully heterologously expressed in S.albus J1074.These results can lay a foundation for the efficient application of Streptomyces nanocompartmental proteins in the synthesis of antibiotics.
万方数据
维普
