摘要
目的 建立同时测定诃子果实、果核中柯里拉京(corilagin)、诃黎勒酸(chebulagic acid)、诃子酸(chebulinic acid)、鞣花酸(ellagic acid)4种鞣质类成分含量的方法.方法 采用Agilent Zorbax C18色谱柱(250 mm×4.6 mm,5 µm),流动相为甲醇(A)-0.1％磷酸(B),梯度洗脱;流速:1.0 ml/min;检测波长:270 nm;柱温:25 ℃.结果 诃子果实、果核中4种成分分离度良好,柯里拉京、诃黎勒酸、诃子酸、鞣花酸在0.0813～5.9936,0.0311～11.4822,0.3271～4.9008,0.0760～1.4935 μg范围内与峰面积呈良好的线性关系,线性方程分别为Y=1915X+336.9,Y=1532.1X+554.89,Y=2077.9X-116.96,Y=7194.4X-53.568;精密度、重复性良好,RSD均小于5％;供试品溶液在室温条件下12 h内稳定;果实和果核4种鞣质类成分平均加样回收率分别在99.73％～102.54％和99.34％～103.32％之间,RSD分别为0.10％～0.86％和0.93％～1.97％;果实、果核中诃黎勒酸含量差异最为显著.结论 该方法简便、准确,重复性好,可用于同时测定诃子果实和果核中柯里拉京、诃黎勒酸、诃子酸、鞣花酸4种成分.
Abstract
Objective To establish a method for simultaneous determination of four tannins(corilagin,chebulagic acid,chebulinic acid and ellagic acid)in the fruits and kernels of Chebulae Fructus.Methods Agilent Zorbax C18 column(250 mm×4.6 mm,5 μm)was used,the mobile phase was methanol(A)-0.1％phosphoric acid(B),and the gradient elution was performed at a flow rate of 1.0 ml/min.The detection wavelength was set at 270 nm,and the column temperature was 25 ℃.Results The four components in the fruits and kernels of Chebulae Fructus were well separated.Corilagin,chebulagic acid,chebulinic acid,and ellagic acid showed good linear relationships in the ranges of 0.0813-5.9936,0.0311-11.4822,0.3271-4.9008 and 0.0760-1.4935 μg,respectively.The linear equations were Y=1915X+336.9,Y=1532.1X+554.89,Y=2077.9X-116.96 and Y=7194.4X-53.568,respectively.The results of precision and repeatability tests were good,with RSDs all less than 5％.The test solution was stable within 12 h at room temperature.The average recoveries of four tannins in fruits and kernels were 99.73％-102.54％and 99.34％-103.32％,respectively,with RSDs of 0.10％-0.86％and 0.93％-1.97％,respectively.The difference between the contents of chebulagic acid in fruits and kernels was the most significant.Conclusion The method is simple,accurate and reproducible.It can be used for the simultaneous determination of corilagin,chebulagic acid,chebularic acid and ellagic acid in the fruits and kernels of Chebulae Fructus.
维普
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