Quality Evaluation of Refined Honey in Gengnianning
Objective To determine fructose,glucose,sucrose,maltose and 5-hydroxymethylfurfural(5-HMF)in Gengnianning to establish a method for evaluation of the quality of refined honey in Gengnianning.Methods The separation was performed on a Prevail Carbohyrate ES column with the refractive index detector,using acetonitrile-water(75∶25)as mobile phase for determination of the carbohydrate components.The Agilent ZORBAX SB-C18 column with UV detector(284 nm)was used for determination of 5-HMF,using acetonitrile-0.1％phosphoric acid(5∶95)as mobile phase.The column temperature was set at 40 ℃ and 30 ℃,respectively,and the flow rate were all 1 ml/min.Results The methodological investigation results of fructose,glucose,sucrose,maltose and 5-HMF all met the requirements.The total content of fructose and glucose and the contents of sucrose,maltose and 5-HMF in 44 batches of Gengnianning samples were 10.3％-36.2％,0.6％-4.6％,0-11.9％,0.01％-0.44％,respectively.There were obvious differences in the samples detection results of carbohydrate components and 5-HMF in Gengnianning.Based on the standard in Chinese Pharmacopoeia(2020 Edition),the quality limits of carbohydrate components and 5-HMF in Gengnianning were formulated.The results showed that 11 out of 44 samples exceeded the limits.Conclusion This method is simple and accurate,and can be used for the quality evaluation of refined honey in Gengnianning.
万方数据
维普
