Objective To establish a method for determination of L-carnitine in health food by solid phase extraction and high performance liquid chromatography.Methods The samples were dissolved and diluted with 10 mmol/L hydrochloric acid solution,and purified by MCX mixed cationic solid phase extraction column,then were separated by ZORBAX SB-AQ C18(250 mm×4.6 mm,5 μm)column,using phosphate buffer solution(consisted of 0.05 mol/L dipotassium phosphate and 0.002 mol/L sodium octane sulfonate adjusted to pH 2.5 with phosphoric acid)-acetonitrile=98∶2 as the mobile phase.The flow rate was 1.0 ml/min,the detection was carried out by UV detector,the detection wavelength was set at 210 nm,and the column temperature was 30 ℃.The qualitative analysis was performed based on retention time,and the quantitative analysis was carried out through external standard method.Results After the sample solution was purified,the interfering substances that could not be separated from the target compound at baseline were effectively removed.The peak area showed a good linear relationship with the mass concentration of L-carnitine in the range of 52.35-418.80 μg/ml,and the correlation coefficient(r)was 0.9996.The average recovery was 91.71％,with RSD of 2.78％.Conclusion The method is reliable,reproducible and can be used for the determination of L-carnitine in health food.
health foodL-carnitinesolid phase extraction(SPE)high performance liquid chromatography(HPLC)
维普
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