卵清蛋白诱导的过敏性鼻炎豚鼠模型的建立与评价及蒙医舌尿诊的初步分析
Establishment and evaluation of ovalbumin induced allergic rhinitis guinea pig model and preliminary anal-ysis of Mongolian medicine tongue and urine diagnosis
乌日柴夫 1苏日古格 1孟永梅1
作者信息
- 1. 内蒙古医科大学 蒙医药学院,内蒙古 呼和浩特 010110
- 折叠
摘要
目的 建立过敏性鼻炎(allergic rhinitis,AR)豚鼠模型并评价,对该模型进行蒙医舌、尿诊分析.方法 将无特定病原体级豚鼠随机分为空白组和模型组,模型组豚鼠给予卵清蛋白(ovalbumin,OVA)与氢氧化铝联合致敏建立AR豚鼠模型;通过豚鼠鼻部症状进行行为学评分,被动皮肤过敏试验判定豚鼠过敏反应程度,ELISA检测豚鼠外周血卵清蛋白特异性IgE(OVA-sIgE)水平,苏木素-伊红(hematoxylin-eosin,HE)染色观察豚鼠鼻黏膜、肺组织病理形态改变进行模型评价;探索性观察豚鼠的舌象,尿液的颜色、泡沫、浮皮、尿渣.结果 与空白组相比,模型组豚鼠滴鼻后打喷嚏、挠鼻次数及流涕等行为学评分显著升高(P<0.01),被动皮肤过敏试验中红肿直径显著增大(P<0.01),OVA-sIgE水平升高(P<0.05),鼻黏膜HE染色出现纤毛受损、黏膜上皮脱落、黏膜下间质水肿、炎性细胞浸润等,肺组织HE染色呈现肺泡壁增厚、气管黏液分泌物增多、少量炎细胞浸润;与空白组相比,模型组豚鼠舌质红、有较明显的红色斑点、舌苔薄,尿液为深黄色并有棕色的分层、少量泡沫、具有形状大小均匀的浮皮或较厚的浮皮、淡黄色较厚尿渣.结论 通过OVA与氢氧化铝联合致敏,结合宏观表征和实验室指标综合评价AR豚鼠模型,结果证明该方法可成功建立AR豚鼠模型,具有可行性.亦可通过蒙医舌、尿诊初步分析AR模型中舌象、尿液的变化,为今后的蒙医模型评价提供实验依据.
Abstract
Objective To establish and evaluate a guinea pig model of allergic rhinitis(AR)and perform Mongolian medicine tongue and urine diagnosis using this model.Methods SPF guinea pigs were randomly divided into control and model groups.To establish the AR guinea pig model,the individuals in the model group were intraperitoneally administered ovalbumin(OVA)com-bined with adjuvant aluminum hydroxide for basic sensitization and a nasal drip to enhance sensitization.Behavioral scoring was con-ducted by observing the nasal symptoms of guinea pigs;the degree of allergic reaction was determined using a passive cutaneous an-aphylaxis test;the level of ovalbumin specific IgE(OVA-sIgE)in the peripheral blood was determined using ELISA;the pathologi-cal morphological changes in the nasal mucosa and lung tissue were observed using hematoxylin eosin(HE)staining.The image of the tongue,as well as the skin,urine color,foam,and urine residue of the guinea pigs were observed.Results Compared with those of the control group,the behavioral scores of sneezing,nasal scratching,and runny nose in the model group guinea pigs in-creased significantly after the nasal drip(P<0.01);the PCA test revealed a significant increase in the diameter of redness and swell-ing(P<0.01)and the level of OVA-sIgE(P<0.05).HE staining of the nasal mucosa revealed cilia damage,mucosal epithelial de-tachment,submucosal interstitial edema,and inflammatory cell infiltration;HE staining of the lung tissue showed thickening of the alveolar wall,increased secretion of tracheal mucus,and a modicum of inflammatory cell infiltration.Compared with those of the control group,the tongues of guinea pigs in the model group showed redness,with obvious red spots,and a thin coating;the urine was dark yellow,with brown layers and without foam,and a light-yellow thick urine residue was observed;and the skin was of uni-form shape and size or thickened.Conclusion An AR guinea pig model can be successfully established using OVA combined with adjuvant aluminum hydroxide sensitization.Macroscopic characterization and laboratory indicators are required to comprehensively e-valuate the AR model.This method is feasible and can provide an experimental reference for the dialectical classification of AR mod-els in the future through Mongolian tongue and urine diagnosis analysis.
关键词
蒙医/卵清蛋白/过敏性鼻炎/豚鼠/模型Key words
Mongolian medicine/Ovalbumin/Allergic rhinitis/Guinea pigs/Model引用本文复制引用
基金项目
国家民委-教育部蒙医药研发工程重点实验室开放基金(MDK2020015)
内蒙古医科大学高等学校教学名师计划(第十五届)(NYJXMS202203)
内蒙古医科大学善学计划(ZY0201031)
内蒙古医科大学中医学(蒙医学)一流学科建设项目(myxylxk202313)
内蒙古医科大学中医学(蒙医学)一流学科研究生创新项目(2022MYYLXKYC006)
内蒙古自治区研究生教学改革项目(JGCG2023122)
出版年
2024
NETL
NSTL
维普
万方数据