单细胞测序分析喉鳞状细胞癌细胞RPN2表达模式
Single cell sequencing analysis of RPN2 expression pattern in laryngeal squamous cell carcinoma cells
吴彬 1周敬淳1
作者信息
- 1. 暨南大学第二临床医学院 耳鼻咽喉科,广东 深圳 518020
- 折叠
摘要
目的 探讨核糖体结合糖蛋白Ⅱ(ribophorin Ⅱ,RPN2)在喉鳞状细胞癌单细胞微环境中的表达模式.方法 通过获取并预处理GSE150321 的喉鳞癌单细胞数据集,应用降维和分群算法对细胞类型进行分类和定义,并对各细胞类型中的RPN2表达进行概览和分析.筛选出RPN2 阳性和RPN2 阴性肿瘤细胞的差异表达基因,进行功能富集分析,构建RPN2 阳性和RPN2 阴性肿瘤细胞的细胞互作网络.结果 喉鳞状细胞癌细胞经单细胞测序后,根据细胞的标记分子将细胞分为肿瘤细胞、免疫细胞、成纤维细胞、内皮细胞和上皮细胞等亚群.RPN2 在肿瘤细胞和免疫细胞中的表达水平相对较高,而在内皮细胞、成纤维细胞和上皮细胞中的表达水平相对较低.差异表达分析筛选出与RPN2 表达相关的基因,功能富集分析结果显示RPN2 阳性肿瘤细胞高表达基因主要富集在核糖核蛋白复合体的生物发生和胞质翻译等信号通路中.构建的细胞互作网络显示RPN2 阳性肿瘤细胞与成纤维细胞、内皮细胞和免疫细胞之间存在较强的互作关系.结论 本研究揭示了RPN2 在喉鳞状细胞癌微环境中的表达模式,以及RPN2 阳性和RPN2 阴性肿瘤细胞的特征及其与肿瘤发病、发展、预后之间的关联.这些发现为喉鳞状细胞癌的治疗和进一步研究提供了理论依据.
Abstract
Objective This study aims to explore the expression pattern of RPN2 in the microenvironment of laryngeal squamous cell carcinoma at the single-cell level.Methods By acquiring and preprocessing the GSE150321 dataset of laryngeal squamous cell carcinoma single-cell data,dimensionality reduction and clustering algorithms were applied to classify and define cell types,fol-lowed by an overview and analysis of RPN2 expression in each cell type.Differential expression genes between RPN2-positive and RPN2-negative tumor cells were screened and subjected to functional enrichment analysis.A cell-cell interaction network was con-structed for RPN2-positive and RPN2-negative tumor cells.Results After single-cell sequencing of laryngeal squamous cell carcino-ma cells,cells were classified into subgroups such as tumor cells,immune cells,fibroblasts,endothelial cells,and epithelial cells based on cell marker molecules.RPN2 showed relatively higher expression levels in tumor cells and immune cells,while lower expression levels were observed in endothelial cells,fibroblasts,and epithelial cells.Differential expression analysis identified genes associated with RPN2 expression,and functional enrichment analysis revealed that highly expressed genes in RPN2-positive tumor cells were mainly enriched in biological processes such as ribonucleoprotein complex biogenesis and cytoplasmic translation signaling pathways.The constructed cell-cell interaction network demonstrated strong interactions between RPN2-positive tumor cells and fibroblasts,endothelial cells,and immune cells.Conclusion This study reveals the expression pattern of RPN2 in the microenvi-ronment of laryngeal squamous cell carcinoma and the characteristics of RPN2-positive and RPN2-negative tumor cells,as well as their associations with tumor occurrence,development,and prognosis.These findings provide a theoretical basis for the treatment and further research of laryngeal squamous cell carcinoma.
关键词
喉鳞状细胞癌/单细胞测序/核糖体结合糖蛋白Ⅱ/微环境/细胞互作网络Key words
Laryngeal squamous cell carcinoma/Single-cell sequencing/Ribophorin Ⅱ/Microenvironment/Cell interactome network引用本文复制引用
出版年
2024
NETL
NSTL
万方数据