KRT4在喉癌及癌旁正常黏膜组织中的表达及临床意义
Expression and clinical significance of KRT4 in laryngeal carcinoma and adjacent normal mucosal tissues
王靖淞 1余灿 1张西 1邓启成 1谢卓良 1赵锐 1温蓓 2刘海1
作者信息
- 1. 川北医学院附属医院 耳鼻咽喉头颈外科,四川 南充 637000
- 2. 四川省人民医院 耳鼻咽喉头颈外科,四川 成都 610031
- 折叠
摘要
目的 探讨角蛋白 4(keratin 4,KRT4)在喉癌患者中的表达水平与临床病理特征及预后之间的关系.方法 收集喉癌组织标本 70 例、对应癌旁正常黏膜组织标本 70 例和声带息肉组织标本 40 例,采用免疫组化SP法和统计学分析KRT4 表达与喉癌的临床病理特征及预后之间的关系.结果 免疫组化SP法显示,KRT4 在喉癌组织的表达水平明显低于癌旁正常黏膜组织、声带息肉组织,差异具有统计学意义(χ2=51.511,P<0.001).KRT4 的表达水平与患者的临床分期(χ2=5.741,P=0.017)、分化程度(χ2=6.713,P=0.010)以及颈部淋巴结转移(χ2=4.639,P=0.031)之间存在显著的相关性.单因素Log-rank检验分析显示,KRT4 表达(χ2=23.962,P<0.001)、临床分期(χ2=4.621,P=0.032)、颈部淋巴结转移(χ2=7.549,P=0.006)与喉癌预后相关,Kaplan-Meier生存曲线提示KRT4 阳性表达组的存活率明显高于KRT4 阴性表达组.Cox回归模型研究发现KRT4 表达是影响喉癌预后的独立危险因素.结论 KRT4 在喉癌组织中的表达水平明显低于癌旁正常黏膜组织、声带息肉组织,且KRT4 表达与患者的临床分期、分化程度及颈部淋巴结的转移有关,KRT4 阳性表达的喉癌患者预后更好.因此,KRT4 有望成为一种重要的生物学指标,用以指导喉癌的诊断、治疗以及预后评估.
Abstract
Objective To investigate the relationship between the expression level of keratin 4(KRT4)in laryngeal cancer patients and the clinicopathological characteristics and prognosis of the patients.Methods 70 specimens of laryngeal cancer tissue,70 speci-mens of normal mucosa tissue adjacent to cancer,and 40 specimens of vocal cord polyp tissue were collected.Immunohistochemical SP method was used,and the relationship between KRT4 expression and clinical pathological characteristics and prognosis of laryn-geal cancer was analyzed statistically.Results The immunohistochemical SP method showed that the expression level of KRT4 in laryngeal cancer tissue was significantly lower than that in adjacent normal mucosal tissue and vocal cord polyp tissue,and the difference was statistically significant(χ2=51.511,P<0.001).There were significant correlationbetween the expression level of KRT4 and the clinical stage(χ2=5.741,P=0.017),degree of differentiation(χ2=6.713,P=0.010),and cervical lymph node metas-tasis of patients(χ2=4.639,P=0.031).Single factor Log rank test analysis showed that KRT4 expression(χ2=23.962,P<0.001),clinical stage(χ2=4.621,P=0.032),cervical lymph node metastasis(χ2=7.549,P=0.006),and prognosis of laryngeal cancer were correlated.The Kaplan Meier survival curve showed that the survival rate of the KRT4 positive expression group was significantly higher than that of the KRT4 negative expression group.Further research using Cox regression models revealed that KRT4 expres-sion is an independent risk factor affecting the prognosis of laryngeal cancer.Conclusion The expression level of KRT4 in larynge-al cancer tissue is significantly lower than that in normal mucosa tissue and vocal cord polyp tissue adjacent to the cancer,and the expression of KRT4 is related to the clinical stage,differentiation degree,and cervical lymph node metastasis of patients.Laryngeal cancer patients with positive expression of KRT4 have a better prognosis.Therefore,KRT4 is expected to become an important bio-logical indicator to guide the diagnosis,treatment,and prognosis evaluation of laryngeal cancer.
关键词
喉癌/角蛋白4/免疫组化/颈部淋巴结转移/Cox回归模型/Kaplan-Meier生存曲线Key words
Laryngeal cancer/Keratin 4/Immunohistochemistry/Cervical lymph node metastasis/Cox regression models/Kaplan-Meier survival curve引用本文复制引用
基金项目
四川省医学青年创新科研课题(S19055)
出版年
2024
NETL
NSTL
万方数据