Objective The aim of this study was to identify small-molecule drugs that inhibit neomycin-induced hair-cell-like cell(HEI-OC1)death,and to verify their effects on hair cells in cochlear explants and the lateral line of zebrafish.Methods Small-molecule drugs that increased the viability of HEI-OC1 cells were identified using Cell Counting Kit-8.The protective effect of the identified small-molecule drugs was verified in hair cells of cochlear explants and zebrafish by Immunofluorescence staining.Results Of the 350 small-molecule drugs screened,clomipramine hydrochloride was found to significantly improve the survival rate of neomycin-treated HEI-OC1 cells.In addition,clomipramine hydrochloride was found to have a protective effect on hair cells of neomycin-treated mouse cochlear explants and the lateral line of zebrafish.Conclusion Clomipramine hydrochloride,which as an FDA-approved clinical drug,has a protective effect against neomycin-induced hair cell damage.
NETL
NSTL
万方数据
