Objective The aim of this study is to investigate R-spondin3(Rspo3)up-regulates the expression of Ncad(N-cadherin,gene named Cdh2)and participates in mouse liver injury.Methods The expression of Rspo3,leucine-rich repeat-containing G protein-coupled receptor 5(Lgr5)and Cdh2 were measured by reverse transcription-quantitative polymerase chain reaction(RT-qPCR).The expression of Ncad was measured by Western blotting and Immunofluorescence.Serum-starved primary cultured mouse bone marrow mesenchymal stromal cell(BMSC)were transfected with Lgr5 siRNA to verify whether Rspo3 up-regulates Ncad expression by acting on Lgr5.Results The Rspo3 and Lgr5 mRNA expression was up-regulated in the liver tissue of mouse injured liver model.The mRNA expression of Cdh2 was up-regulated and positively correlated with the expression of Rspo3 and Lgr5,which is one of the receptors of Rspo3.Immunofluorescence shows that Ncad is mainly localized in the pericytes of the mouse injured liver.The Rspo3-induced up-regulation of Ncad was inhibited after Lgr5 siRNA transfection.Conclusion In the mouse injured liver tissue,Rspo3 up-regulates the expression of Ncad in pericytes by acting on Lgr5,and affects mouse liver injury.
liver injuryN-cadherinR-spondin3leucine-rich repeat-containing G protein-coupled receptor 5
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NSTL
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