Objective To investigate the anti-fatigue effects and acute toxicity of different doses of D-borneol refreshing atomization solution on mice,and to provide reference for the further development and application of D-borneol refreshing atomization solution in clinic.Methods Sixty specifc pathogen free(SPF)male KM mice were divided into one blank group and four dose groups,with 12 mice in each group,for acute toxicity experiments and weight-bearing swimming experiments.In the acute toxicity test,the toxic reaction of mice was observed.After dissection,histopathological examination was performed.For weight-bearing swimming experiment,serum,liver and hind leg muscle tissues of mice were collected.Blood glucose(Glu),lactic acid(LA),blood urea nitrogen(BUN),and lactate dehydrogenase(LDH)activity levels were measured in serum.Liver glycogen(LG)content was assessed in liver tissue;muscle glycogen(MG)and other anti-fatigue biochemical indicators were evaluated in muscle tissue.Malondialdehyde(MDA)content and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were determined in muscle tissue.Results During the administration of D-borneol refreshing atomization solution,no abnormalities were observed in the mental state,fur color,or body weight of mice across all groups during the acute toxicity test.Pathological examinations did not reveal any abnormalities either.The weight-bearing swimming test results demonstrated that medium and high doses of D-borneol refreshing atomization solution could effectively prolong the time of weight-bearing exhaustive swimming in mice(P<0.001),significantly increase the content of MG(P<0.01),and reduce the activities of serum LD and LDH(P<0.05).The serum BUN level was decreased(P<0.01),the blood glucose level was increased(P<0.01),and the serum MDA(malondialdehyde)level was decreased(P<0.05).The levels of SOD(superoxide dismutase)(P<0.01)and GSH-Px(glutathione peroxidase)(P<0.001)were increased.Conclusion D-borneol refreshing atomization solution has no obvious toxic and side effects,and has good refreshing and anti-fatigue effects.The mechanism may be related to improving antioxidant capacity and reducing muscle damage.
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