The effect of HI-TOPK-032 on the proliferation of ovarian cancer based on a nude mouse model
Objective To investigate the expression of T-LAK cell-originated protein kinase/PDZ-binding-kinase(TOPK/PBK)in ovarian cancer and the effect of HI-TOPK-032,an inhibitor of TOPK/PBK,on ovarian cancer cells.Methods The expression of TOPK/PBK in ovarian cancer tissue and normal samples were analyzed by gene expression profiling interactive analysis(GEPIA)software.The effect of HI-TOPK-032 on the proliferative activity of ovarian cancer cells was analyzed by methyl thiazolyl tetrazolium(MTT)assay and colony formation assay.The effect of HI-TOPK-032 was also assessed on subcutaneous ovarian tumor in nude mice.Results The expression of TOPK/PBK in ovarian cancer was significantly higher than that in normal ovarian tissue(P<0.05).With the treatment of HI-TOPK-032,the expression of TOPK/PBK was inhibited,and the proliferation capacity of OVCAR3 and SKOV3 cells decreased(P<0.0001).The mass[(1.514±0.358)g vs(0.670±0.450)g]and volume[(0.418±0.171)cm3 vs(0.973±0.262)cm3]of subcutaneous tumors in the HI-TOPK-032 treatment group were significantly lower than that in the control group.Conclusions TOPK/PBK is highly expressed in ovarian cancer,contributing to the growth of ovarian cancer.HI-TOPK-032 exerts an inhibiting effect on the growth of ovarian cancer.
ovarian cancerHI-TOPK-032T-LAK cell-originated protein kinase/PDZ-binding-kinasecell proliferation
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