Exploratory study on the ameliorative effects of Trichinella spiralis paramyosin on chronic inflammatory bowel disease
Objective This investigation seeks to discern the effect of Trichinella spiralis paramyosin(Ts Pmy)in modulating chronic colitis.Methods We commenced with the preparation of Ts Pmy,followed by the induction of chronic colitis in Rag1 KO mice via naïve T cells.The subjects Rag1 KO mice were systematically allocated into three groups by random number table:a treatment group receiving CD4+Foxp3-CD45RBhi T cells with Ts Pmy,a control group receiving CD4+Foxp3-CD45RBhi T cells with PBS,and a baseline group consisting of untreated Rag1 KO mice.Mice diagnosed with chronic colitis received intraperitoneal Ts Pmy injections.Subsequent evaluations included assessments of clinical manifestations,colon shortening,and histological examination of colonic inflammation using hematoxylin-eosin(HE)staining.Flow cytometry facilitated the quantitative analysis of T helper 1 cell(Th1)and T helper 17 cell(Th17)cell populations in the colonic lamina propria,alongside the enumeration of gut-specific CD103+regulatory dendritic cells(DCs).Results Administering Ts Pmy conferred a significant attenuation of the weight loss,disease activity index,and pathological damage of colon tissue in mice.Key findings included a marked decrement in the proportions of pro-inflammatory Th1 and Th17 cells and an enhanced presence of immunomodulatory CD103+DCs within the colonic lamina propria.Conclusions Ts Pmy has a mitigating influence on chronic colitis in murine models through the expansion of CD103+DCs and the concurrent reduction of Th1 and Th17 cell populations.These insights pave the way for further investigation into helminth-derived paramyosins as a novel therapeutic strategy for chronic colitis.
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