Objective:To investigate the methods for distinguishing and identifying the easily confusing Chinese medicinal cortex:Digupi(Lycii Cortex),Xiangjiapi(Periplocae Cortex)and Wujiapi(Acanthopanacis Cortex).Methods:The identification of Digupi,Xiangjiapi and Wujiapi were studied by traits,micro-traits,microscopy and fluorescence identification.Results:The main identification points of Digupi,Xiangjiapi and Wujiapi in terms of traits and micro-traits are the external surface,section and odor.The focus of microscopic identification is the different crystalliza-tion of calcium oxalate.The fluorescence phenomenon of the three herbs observed by traditional fluorescence method under ultraviolet lamp were not significantly different,but there were great differences in two-dimensional fluorescence and three-dimensional fluorescence.The results of two-dimensional fluorescence spectrum showed that there was only one fluorescence peak in the water extract of the three medicinal materials,and the peak position,peak intensity and peak shape of the three medicinal materials were very different.The results of three-dimensional fluorescence spectrum showed that there were three fluorescence peaks in Digupi,two fluorescence peaks in Xiangjiapi and Wujiapi,and the peak position and peak intensity of the three medicinal materials were significantly different.Conclusions:The traditional identification methods can be used for the identification of Digupi,Xiangjiapi and Wujiapi.Two-dimensional fluorescence spectroscopy and three-dimensional fluorescence spectroscopy can quickly and accurately distinguish the three medicinal materials.
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