Distinguishing Between Zhizi(Gardeniae Fructus)and Jiaozhizi(Gardeniae Fructus Praeparatus)Based on Quantitative Evaluation of Microscopic Characteristic Color
Distinguishing Between Zhizi(Gardeniae Fructus)and Jiaozhizi(Gardeniae Fructus Praeparatus)Based on Quantitative Evaluation of Microscopic Characteristic Color
Objective:To establish a method to distinguish Zhizi(Gardeniae Fructus)and Jiaozhizi(Gardeniae Fructus Praeparatus)based on quantitative evalua-tion of microscopic characteristic color.Methods:The microscopic imaging technology and the micro-scopic characteristics color extraction software were used to measure the microscopic characteristic color.Kruska-Wallis H test and Fisher discriminant analysis method were used to analyze the micro-scopic characteristic color differences between Zhizi and Jiaozhizi,and a discriminant function was established.Results:There were significant differ-ences in the lightness value(L*),red-green value(a*),yellow-blue value(b*),total chromaticity values(E*ab)of microscopic characteristics(endocarp cell,endocarp fiber,seed stone cell)between Zhizi and Jiaozhizi(P＜0.01).Zhizi and Jiaozhizi were distinguished by the color of seed stone cells and the discriminant func-tion of microscopic characteristic color:y=0.678 ×L*+0.384 ×a*-0.576 ×b*-9.322,y＞0 corresponding to Zhizi,y＜0 corresponding to Jiaozhizi.Conclusions:The effective discrimination of Zhizi and Jiaozhizi is realized through the quantitative evaluation of microscopic characteristic color,which provides a new method for the discrimination and quality evaluation of raw and processed decoction pieces of Zhizi.
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