Effect of Compound Qinbai Lotion(复方秦柏洗剂)and Tuhuaidan Siwu Decoction(土槐丹四物汤)on Inflammatory Factors in Psoriasis Model Mice
Objective:To observe the effect of Com-pound Qinbai Lotion(复方秦柏洗剂)and Tuhuaidan Siwu Decoction(土槐丹四物汤)on ear swelling inflammation and the granular layer of tail scale epidermis in mice,to provide the basis for the treatment of psoriasis.Methods:Total 48 male KM mice were selected and divided into the blank group,the model group,the calcipotriol ointment group,Compound Qinbai Lotion group,Tuhuaidan Siwu Decoction group,Compound Qinbai Lotion+Tuhuaidan Siwu Decoction group according to the random number table method,with 8 mice in each group.The blank and model groups were treated with saline topically and by gavaged,while the medication groups were treated with their respective drugs topically and by gavaged for 7 consecutive days.One hour after the last administration,20 µL xylene was applied evenly on both sides of the right ear of mice in other groups except the blank group to establish the ear swelling and inflammation model.The degree of ear swelling was then observed.Enzyme linked immunosorbent assay(ELISA)was used to determine the content of interleukin-17(IL-17)and interleukin-23(IL-23)in the serum.Hematoxylin-Eosin(HE)staining was used to observe the pathological changes in ear skin lesions.Immunohistochemistry was used to measure the expressions of interferon-γ(IFN-γ)and interleukin-6(IL-6)in ear tissue.A total of 40 female KM mice were selected and divided into the blank group,calcipotriol ointment group,Compound Qinbai Lotion group,Tuhuaidan Siwu Decoction group,and Compound Qinbai Lotion+Tuhuaidan Siwu Decoction group according to the random number table method,with 8 mice in each group,using the tail scale epidermis model directly.The blank group was treated with saline topically and by gavaged,while the medication groups were treated with their respective drugs topically and by gavaged for 14 consecutive days.The number of the granular layer of tail scale epidermis in mice was measured.ELISA was used to determine the content of IFN-γ and IL-6 in the serum.HE staining was used to observe the formation of the granular layer of tail scaleepid ermis in mice.Immunohistochemistry was used to measure the expressions of IL-17 and IL-23 in tail skin tissue.Results:Compared with the model group,the degree of ear swelling in the male mice of each treatment group reduced(P＜0.01),and the cell number of granular layer of tail scale epidermis in female mice increased(P＜0.01).The serum content of IL-17,IL-23 in the male mice and IFN-γ,IL-6 in the female mice of the Compound Qinbai Lotion+Tuhuaidan Siwu Decoction group significantly decreased(P＜0.01).The expressions of IFN-y and IL-6 in the ear tissue of male mice and IL-17,IL-23 in the tail skin tissue of female mice also significantly decreased(P＜0.01).Compared with the calcipotriol ointment group,there was no statistical significance in the above indicators in the Compound Qinbai Lotion+Tuhuaidan Siwu Decoction group(P＞0.05).Conclusions:Compound Qinbai Lotion combined with Tuhuaidan Siwu Decoction can inhibit inflammation and promote normal epidermal differentiation by reducing the expressions of inflammatory factors IL-17,IL-23,IFN-γ and IL-6,thereby playing a role in the treatment of psoriasis.
psoriasisCompound Qinbai LotionTuhuaidan Siwu Decoctionear swellinggranular layer of tail scale epidermis in mousecell numberinflammatory factorsmouse
万方数据
维普
