Effect and Kinetics of Different Solvent Extracts of Zhijuzi(Hovenia Dulcis)on Xanthine Oxidase
Objective:To investigate the effect and kinetic model of Zhijuzi(Hovenia Dulcis)extracted by different solvents on xanthine oxidase(XOD),and to provide experimental evidence for the study of hyperuricemia.Methods:Zhijuzi was extracted with water,50％ethanol,70％ethanol,and 95％ethanol respectively.The content of flavonoid constituents in each extract was measured,and the effect and kinetic model of the different solvent extracts on XOD were analyzed by ultraviolet spectrophotometry.Results:The extracts of Zhijuzi with different solvents all had inhibitory effects on XOD,among which the inhibitory effect of 50％and 70％ethanol extracts were relatively strong,and the contents of flavonoids in the extracts were also higher,and the inhibitory effect was dose-dependent.Although the total amount of flavonoids in 95％ethanol extract was lower,it also showed good inhibitory effect.By kinetic analysis,the affinity value α of different solvent extracts for XOD was greater than 1,and the affinity value of 70％ethanol extract for XOD was 134.40,which was more easier to bind to XOD.All extracts showed mixed inhibition.Conclusions:Zhijuzi extract could inhibit XOD,and the 50％and 70％ethanol extracts were more effective.The 95％ethanol extract was associated with other components in the extract.The type of inhibition of Zhijuzi extract was mixed inhibition,that was,competitive-noncompetitive inhibition,and the competitive inhibition was dominant.
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