Effect of Dayuan Decoction(达原饮)on TLR4/NF-κB/NLRP3 Pathway in Gastric Tissue and Perioral Skin of Model Rats with Hp Infection
Objective:To observe the effect of Dayuan Decoction(达原饮)on the Toll-like receptor 4/nuclear factor-KB/NOD-like receptor protein 3(TLR4/NF-κB/NLRP3)signaling pathway and inflammatory factors such as tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)in the gastric tissue and perioral skin of model rats with Helicobacter pylori(Hp)infection,to explore the mechanism of Dayuan Decoction in treating Hp infection.Methods:Total forty-four male SD rats were randomly divided into the blank group(8 rats)and the modeling group(36 rats).The modeling group was administered Hp bacterial suspension via gastric tube injection to establish the model.After the modeling was established,the modeling group was randomly divided into the model group,the high-dose group of Dayuan Decoction,the low-dose group of Dayuan Decoction,and the Western medicine group,with 8 rats in each group.The blank group and the model group were administered an equal volume of normal saline via gastric tube injection,and the rats in other group were administered the corresponding drugs via gastric tube injection for 2 weeks.The changes in the general condition of the rats before and after the modeling were observed.Hematoxylin-eosin(HE)staining was used to observe the pathological changes in the perioral skin and gastric tissue,and the enzyme linked immunosorbent assay(ELISA)was used to detect the expression of TLR4,NF-κB,NLRP3 in the perioral skin and gastric tissue of the rats and the expression levels of TNF-α and IL-1β in the serum.Results:After drug intervention,compared with the model group,the rats in the Western medicine group,high-dose group of Dayuan Decoction,and low-dose group of Dayuan Decoction had soft and shiny fur,increased appetite and water intake,gradually formed stool,and the epithelial cells of the gastric mucosa had clear layers and were arranged relatively neatly;The infiltration of inflammatory cells was reduced,and the conditions of keratinization overgrowth,thickening of the stratum corneum,and mild infiltration of inflammatory cells around the hair follicles in the perioral skin were all improved to varying degrees.Compared with the model group,the expression levels of TLR4,NF-KB,and NLRP3 proteins in the gastric tissue and perioral skin of the rats in the Western medicine group,high-dose group of Dayuan Decoction,and low-dose group of Dayuan Decoction were all downregulated to varying degrees(P＜0.05 or P＜0.01),and the expression of inflammatory factors such as TNF-α and IL-1β in the serum was significantly downregulated(P＜0.01).Conclusions:By intervening TLR4/NF-κB/NLRP3 signaling pathway,Dayuan Decoction may inhibit the expression of TLR4,NF-κB,NLRP3 and downstream inflammatory factors,reduce the gastric tissue and perioral skin inflammatory damage in Hp infected rats.
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