Study on Mechanism of Three-Dimensional Balanced Spine Manipulation Combined with Acupuncture Intervention in Model Rats with Intervertebral Disc Degeneration
Objective:To investigate the mechanism of three-dimensional balanced spine manipulation combined with acupuncture in model rats with intervertebral disc degeneration(IVDD).Methods:Thirty-six SD rats were divided into sham operation group,model group,acupuncture group and combined treatment group according to the random number table method,with 9 rats in each group.The IVDD model was established by urokinase-type fibrinogen activator(uPA)injection in the model group,acupuncture group and combined treatment group,and the soft tissues of rats in the sham operation group were cut according to the modeling procedure,but not injected with uPA.After the successful modeling,the model group and the sham operation group did not receive any intervention,the acupuncture group was treated with needling,and the combined treatment group was treated with three-dimensional balanced spine manipulation combined with needling,the intervention lasted for 6 weeks.At the end of the experiment,we observed the morphological changes of the target intervertebral discs in rats and detected the expression levels of interleukin-1 β(IL-1β)protein and proteoglycan,type Ⅱ collagen(Col2A1)and matrix metalloproteinase-3(MMP-3)mRNA.Results:The morphology of the intervertebral discs of rats in each group showed some differences.Hematoxylineosin staining(HE staining)showed that the nucleus pulposus and annulus fibrosus of the intervertebral discs in the sham operation group were the most intact,while the model group had no nucleus pulposus,and the annulus fibrosus of the model group had a lot of radial rupture traces,and the annulus fibrosus of the acupuncture group and the combined treatment group had a few rupture traces and preserved nucleus pulposus,while the structure of the intervertebral disc in the combined treatment group was more complete.Compared with the sham operation group,the protein expression level of IL-1β in the model group was increased significantly(P＜0.05),proteoglycan and Col2A1 mRNA expression levels were decreased significantly(P＜0.05),and the expression level of MMP-3 mRNA was significantly raised(P＜0.05).Compared with the model group,IL-1β protein expression level was lowered in the acupuncture group and combined treatment group(P＜0.05),proteoglycan and Col2A1 mRNA expression levels were raised(P＜0.05).MMP-3 mRNA expression level was reduced in the acupuncture group,there was no statistical significance(P＞0.05);MMP-3 mRNA expression level was decreased significantly in the combined treatment group(P＜0.05).Compared with the acupuncture group,IL-1β protein expression level was reduced significantly in the combined treatment group(P＜0.05),proteoglycan and Col2A1 mRNA expression levels were raised significantly(P＜0.05),and MMP-3 mRNA expression level was decreased significantly(P＜0.05).Conclusions:①uPA injection can induce IVDD in rats.②The expression levels of IL-1β,MMP-3,Col2A1 and proteoglycan were altered in the intervertebral discs of rats in the IVDD model,and IL-1β,MMP-3,Col2A1 and proteoglycan could be involved in intervertebral disc degeneration.③Three-dimensional balanced spine manipulation combined with acupuncture can promote the morphological recovery of intervertebral discs in IVDD model rats,and can intervene in intervertebral disc degeneration by regulating the expression levels of IL-1β,MMP-3,Col2A1 and proteoglycan.④The intervention effect of three-dimensional balanced spine manipulation combined with acupuncture on intervertebral disc degeneration was superior to that of acupuncture intervention alone.
万方数据
维普
