Quality Standard and Antioxidant Activity of Xueju(Coreopsis Tinctoria)
Objective:To establish a quality inspection method for Xueju(Coreopsis Tinctoria),and evaluate the antioxidant activity of Xueju from different origins.Methods:The morphological and microscopic identifi-cation of 12 batches of Xueju were carried out.The content of moisture,total ash and extract was determined by referring to Pharmacopoeia of the People's Republic of China(2020 edition),and the total flavonoid content was determined by ultraviolet-visible spectrophotometery(UV-Vis).The 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radical clearance rate of 12 batches of Xueju and its effect on serum superoxide dismutase(SOD)activity and malondialdehyde(MD A)concentration in mice were measured.The antioxidant activity of Xueju from different origins was evaluated by analytic hierarchy process-coefficient of variation-technique for order preference by similarity to an ideal solution.Results:The traits of 12 batches of Xueju were stable.The moisture content and total ash content were(6.920±0.02)％～(9.71±0.12)％and(5.90±0.03)％～(7.46±0.21)％,respectively.The water-soluble and alcohol-soluble extracts were(38.63±0.30)％～(45.78±0.07)％and(40.77±0.14)％～(46.55±0.24)％,respectively,and the total flavonoid content ranged from(168.44±1.27)～(289.42±0.25)mg/g.The antioxidant activity of 12 batches of Xueju was ranked as follows:S1(Keliyang,Xinjiang)＞S8(Lijiang,Yunnan)＞S6(Dulan,Qinghai)＞S2(Korla,Xinjiang)＞S7(Kunming,Yunnan)＞S12(Lhasa,Tibet)＞S5(Xining,Qinghai)＞S9(Lijiang,Yunnan)＞S3(Keliyang,Xinjiang)＞S4(Xining,Qinghai)＞S11(Linzhi,Tibet)＞S10(Linzhi,Tibet).Conclusions:It was preliminarily determined that the moisture and total ash content of Xueju should not exceed 12％and 8％.The water-soluble extract and alcohol-soluble extract should not be less than 30％,and the total flavonoid content of Xueju should not be less than 160 mg/g.The antioxidant activities of Xueju from S1(Keliyang,Xinjiang),S8(Lijiang,Yunnan)and S6(Dulan,Qinghai)were better.
Xueju(Coreopsis Tinctoria)morphological identificationmicroscopic identificationcontent determi-nationantioxidant activityanalytic hierarchy process-coefficient of variation-technique for order preference by similarity to an ideal solution
万方数据
维普
