Optimization of Extraction Process of Hippophae Fhamnoides L. Polysaccharide by Re-sponse Surface Methodology
Objective:To optimize the extraction process of Hippophae fhamnoides L. polysaccharide by re-sponse surface methodology. Methods:Based on the single-factor experiment,the influence of extraction ten-perature,extraction time and ratio of water to material on extraction yield of Hippophae fhamnoides L. polysaccharide was study by response surface methodology. Results:The optimum extraction conditions of Hippophae fhamnoides L. polysaccharide were as follows: the extraction temperature was 97.2℃,the extrac-tion time was 60.3 minutes and the ratio of water to material was 15.65∶1(mL/g). Under this condition,the yield of polysaccharide from Hippophae fhamnoides L. Was 10.28%. Conclusion:The response surface methodology is simple and with high yield,so it can be used to extract polysaccharide from Hippophae fhamnoides L.