摘要
目的:探讨督灸对气道高反应(AHR)患者炎症水平、肠道菌群及生活质量的影响.方法:采用自身前后对照试验,纳入30例AHR患者,采用督灸干预,观察患者炎症因子白细胞介素-4(IL-4)、白细胞介素-17(IL-17)、肿瘤坏死因子-α(TNF-α)水平,肠道菌群结构、丰度、多样性、特征属,以及生活质量情况[采用圣乔治呼吸问卷(SGRQ)评估].结果:接受督灸干预4周的AHR患者IL-4、IL-17、TNF-α水平、索伦森(Sorensen)指数、拟杆菌属丰度均有好转,差异均有统计学意义(P＜0.05).督灸干预前患者的关键细菌主要为脱硫弧菌、单形拟杆菌、革兰阳性菌、丝状霍尔德菌、格氏乳球菌、乳酸杆菌、甲酸芽殖菌、普氏菌、罗氏菌、乳酸乳球菌和S24_7菌,督灸干预后的关键细菌主要为多尔氏菌、伶俐瘤胃球菌、摩氏摩根菌、沙雷菌、殊异韦荣球菌、薰衣草梭菌、螺旋藻梭菌、阿尔登梭菌和松鼠葡萄球菌.督灸疗程结束后,患者SGRQ评分低于督灸干预前,差异具有统计学意义(P＜0.05).结论:督灸能改善AHR患者临床症状和生活质量,其作用机制可能与抑制炎症反应、调节肠道菌群有关.
Abstract
Objective:To investigate the effects of Du-moxibustion on inflammation level,intestinal flora and quality of life in patients with airway hyperresponsiveness(AHR).Methods:A self-controlled before and after trial was conducted in 30 patients with AHR,the intervention of Du-moxibustion was given.The levels of inflammatory factors[interleukin-4(IL-4),interleukin-17(IL-17),tumor necrosis factor-α(TNF-α)],intestinal flora structure,abundance,diversity,characteristics and quality of life[assessed by applying St.George's Respiratory Questionnaire(SGRQ)]of the patients were observed.Results:The levels of IL-4,IL-17,TNF-α,Sorensen index,and abundances of Bacteroides in AHR patients receiving Du-moxi-bustion intervention for 4 weeks were improved,which were with statistically significant differences(P＜0.05).The key bacteria of the patients before Du-moxibustion intervention were Vibrio desulphuricoides,Bacteroides monoformis,Gram-positive bacteria,Holderia filamenti,Lactococcus grigeri,Lactobacillus lactobacilli,Bacillus formicum,Prevotella,Roche,Lactococcus lactis and S24_7 bacteria.The key bacteria after Du-moxibustion intervention were Doleria,Rumenococcus limeniae,Rhizobacteria mosieri,Serratia,Veillonella variabilis,Clostridium lavandulae,Clostridium spiralis,Clostridium aldendii and Staphylococcus sciuris.The SGRQ scores of AHR patients after Du-moxibustion intervention were significantly lower than those before the intervention,and the difference was statistically significant(P＜0.05).Conclusion:Du-moxibustion intervention can relieve the clinical symptoms and improve the quality of life in patients with AHR,and its mechanism may be related to inhibiting inflammation and regulating intestinal flora.
基金项目
江苏省中医药科技发展计划项目(YB2020025)
江苏省科技攻关计划(BE2023786)
第四批江苏省中医临床优秀人才研修项目(苏中医科教[2022]1号)
维普
万方数据