Composition and drug resistance of pathogenic bacteria in human eye infection in Shandong Province between 2017 and 2022
Objective To explore the composition and drug resistance of ocular pathogens isolated from different popu-lations in Shandong,and to provide reference for clinical antimicrobial drug selection.Methods In this retrospective analysis,731 strains of pathogenic bacteria were collected,which were from patients with eye infection reported by members of the Shandong Province Pediatric Antimicrobial Resistance Surveillance System(52 tertiary hospitals and 7 secondary hospitals)between Jan.2017 and Dec.2022.The source sites included conjunctival sac secretions,palpebral margin secretions,lacrimal sac secretions,corneal scrape and vitreous puncture.The isolated strains were identified with mass spectrum system,automatic microbial identification system and API system.The drug sensitivity test methods rec-ommended by the American Association for Clinical and Laboratory Standards were performed and the results were in-terpreted.The data were analyzed with WHONET 5.6,and the differences in bacterial resistance were analyzed with SPSS 21.0.Results ①The main pathogens isolated were gram-positive bacteria(70.2％),among which Staphylococ-cus ranked the first;Staphylococcus aureus was the main pathogen in children,and Staphylococcus epidermidis was the main pathogen in adults and the elderly.The gram-negative bacteria were mainly pseudomonas aeruginosa.Fungal infec-tion occurred mainly in adult males,with Fusarium as the dominant species.② The resistance rates of gram-positive bacteria to vancomycin and linezolid were 0,rifampicin 2.0％,chloramphenicol 4.3％,gentamicin 9.2％,moxifloxacin 10.6％,levofloxacin 33.2％and erythromycin 77.6％.The resistance rate of Staphylococcus to penicillin G was higher than that of streptococcus.The resistance rates of Staphylococcus epidermidis to rifampicin,quinuptin/dafotene,moxi-floxacin,tetracycline,ciprofloxacin,levofloxacin and cotrimoxazole were higher than those of Staphylococcus aureus.The resistance rate of Streptococcus bradycida to levofloxacin was higher than that of Streptococcus pneumoniae.③The resistance rate of gram-negative bacteria to amikacin was 1.7％,tobramycin 8.0％,levofloxacin 11.1％,gentamicin 11.2％and chloramphenicol 18.2％.The resistance rate of Pseudomonas aeruginosa was generally low.Conclusion ①Staph-ylococcus is the main pathogen of infectious eye diseases.② Gram-positive bacteria were generally resistant to erythromycin and penicillin.The drug resistance rate of gram-negative bacteria can be well controlled.In addition to van-comycin and other ultimate drugs,rifampicin can be used to treat ocular gram-positive infection.Amilacin may be consid-ered for gram-negative bacterial infections.③It is necessary to pay attention to and monitor the distribution and change trend of ocular pathogens in different age groups.The use of antibiotics should be standardized according to the results of drug sensitivity.The value of antibiotics that have been rarely used in ophthalmology should also be re-evaluated.
Eye infectionPathogenic bacteriaDrug resistanceShandong Province
NETL
NSTL
万方数据
维普
