Rapid and accurate assessment of pulmonary hypertension in rats via diaphragmatic puncture method
Objective To establish a simple,rapid and accurate method to measure the right ventricular pressure in rats.Methods A total of 30 male SD rats aged 8-10 weeks were randomly divided into 3 groups:the trans-thoracic right ventricular puncture manometry group(TTM group,n= 10),trans-diaphragmatic puncture manometry group(TDM group,n = 10),and the monocrotaline-induced pulmonary hypertension with trans-diaphragmatic manometry group(MCT-TDM group,n=10).The MCT-TDM group underwent subcutaneous injection of monocrotaline through the tho-racoabdominal side(60 mg/kg),while the TTM and TDM groups were injected with an equivalent volume of sterile saline.Four weeks after model establishment,the right ventricular systolic pressure(RVSP)of the TDM and MCT-TDM groups was measured using the trans-diaphragmatic puncture method,followed by thoracotomy to verify the accu-racy of the puncture site;RVSP of the TTM group was measured using the trans-thoracic right ventricular puncture method.The operation time,success rate,and RVSP were compared between the two manometry methods.The hearts and lung tissues of the rats were extracted,left and right ventricles were separated,and the right ventricular hypertrophy index(RVHI)was calculated.Lastly,the left lung tissue was stained with hematoxylin and eosin(HE)to evaluate the extent of pulmonary microvascular lesions.Results The time taken from anesthesia to successful measurement of RVSP was(6.83±1.36)minutes for the TTM group,(8.13±1.25)minutes for the TDM group,and(8.32±1.23)mi-nutes for the MCT-TDM group.The success rate were 70%for the TTM group,100%for the TDM group,and 90%for the MCT-TDM group.There were no significant differences in RVSP and RVHI between the TDM and TTM groups(P>0.05).The RVSP and RVHI in the MCT-TDM group were significantly higher than those in the TDM group(P<0.001).In the MCT-TDM group,the pulmonary arterioles showed notable thickening of the vessel walls and narrowing of the lumens.Conclusion Measuring the right ventricular pressure with diaphragmatic puncture technique negates the need for thoracotomy and circumvents the use of costly equipment.It enables precise,swift,and straightforward assess-ment of the right ventricular hemodynamics,holding considerable value for widespread application.
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