P19 activates the Gq pathway of GPR56 to promote browning of beige adipose
Objective To investigate the influence of GPR56 Stachel peptide(P19)on the browning of beige adipose tissue and its molecular mechanisms.Methods qPCR and Western blotting techniques were used to assess the expres-sion levels of GPR56 in beige adipose tissue.ELISA assay was used to investigate the impact of 50 μmol/L P19 on the downstream IP3 level changes caused by activation of GPR56 Gq pathway in mouse beige adipose tissue.Subsequently,during the induction of browning differentiation in primary beige adipocytes,the expression levels of browning marker genes and thermogenic marker genes were evaluated using qPCR.A comprehensive analysis was performed to elucidate the effects of P19,YM-254890(YM),and Gpr56 gene silencing on the differentiation process.Results GPR56 was highly expressed in beige adipose tissue.P19 can increase the expression levels of browning marker genes(Tbx and Tmem26)and heat production marker genes(Ppargc1a,Cox7a1 and Cidea)with statistically significant differences(P＜0.05).Moreover,when YM was added or Gpr56 expression was silenced,a reversal in the expression levels of these browning and thermogenic marker genes was observed.Conclusion P19 can promote the browning process of beige adipose tissue through the activation of the Gq pathway.
GPR56P19Adhesion G protein-coupled receptorsSignal transduction pathwayAdipose differentiation
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