Secondary metabolites and their activities of Penicillium terrigenum RD 4-3 isolated from saline-alkali soil in the Yellow River Delta
Objective To study the secondary metabolites and biological activities of fungus Penicillium terrigenum RD 4-3 isolated from saline-alkali soil in the Yellow River Delta.Methods The secondary metabolites were isolated and purified using various chromatographic methods from culture broth,and the structures of the pure compounds were iden-tified based on their physicochemical properties and spectral data.Cytotoxic activity and NO detection were performed to explore antineoplastic activity and anti-inflammatory activity.Results Fourteen pure compounds were isolated and identified from the extracts of Penicillium terrigenum RD 4-3,including sesquiterpenes 14-hydroxypetasol(1),6-dehy-dropetasol(2),isopetasol(3),acremeremophilane G(4),phomenone(6),7-hydroxypetasol(7),sporogen-AO1(9),petasol(10),JBIR-28(11),JBIR-27(12),3-acetyl-13-deoxyphome(13)and penicilleremophilane A(14),and benzene derivatives 3-chloro-4-hydroxypheylacetamide(5)and phenols(8),among which 14-hydroxypetasol was a new compound.The activity results showed that phomenone(6)and sporogen-AO1(9)exhibited significant antitu-mor activities against non-small cell lung cancer cells A549 and H1299,with IC50 values below 10 μmol/L.At a con-centration of 50 μmol/L,phomenone,petasol and JBIR-28 exhibited anti-inflammatory activity in the LPS-induced in-flammation model.Preliminary structure-activity relationship revealed that the 6,7-epoxyl structure could significantly increase the antitumor and anti-inflammatory activities of the compounds.Conclusion The sesquiterpenes of the sec-ondary metabolitesfrom fungus Penicillium terrigenum RD 4-3 from the Yellow River Delta exhibit great potential in the discovery of antitumor and anti-inflammatory lead compounds.
Yellow River DeltaFungiPenicillium terrigenum RD 4-3Secondary metabolitesAntitumor activityAnti-inflammatory activity