Subcellular localization pattern of bromodomain containing 9 in colorectal cancer and its clinical significance
Objective To investigate the protein expression level,subcellular localization pattern and clinical signifi-cance of bromodomain containing 9(BRD9)in colorectal cancer(CRC).Methods Surgical resection specimens of CRC were collected,and immunohistochemistry(EnVision method)was used to detect BRD9 protein expression and subcellular localization.The relationship between BRD9 expression and clinicopathological features and prognosis of CRC patients was analyzed.Results Among 508 CRC tissue specimens,BRD9 was overexpressed in 71.46%(363/508)of patients,and 80.31%(408/508)of patients showed nuclear positive staining in immunohistochemistry for BRD9.The expression of BRD9 was correlated with tumor size(P=0.025),lymph node metastasis(P=0.004),nerve invasion(P=0.043),differentiation grade(P=0.017)and TNM stage(P=0.002).The subcellular localization of BRD9 was correlated with lymph node metastasis(P=0.040)and TNM stage(P=0.026).The difference was statisti-cally significant.Meanwhile,BRD9 high expression served as an independent poor prognostic factor(HR=1.994,P=0.002).More important,in patients with BRD9 low expression,cytoplasmic BRD9 expression was an independent poor prognostic factor of patients(HR=8.056,P=0.001).Conclusion The subcellular localization of BRD9 is heterogene-ous in CRC patients.The high expression and cytoplasmic localization of BRD9 are related to the occurrence and devel-opment of CRC,and could be used as an independent predictor for the prognosis of CRC patients.
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