结直肠癌中含溴结构域蛋白9的亚细胞定位模式及其临床意义
Subcellular localization pattern of bromodomain containing 9 in colorectal cancer and its clinical significance
刘春铖 1刘晓晗 1魏闫若雪 1李梓绮 1刘玉昆 2赵然2
作者信息
- 1. 山东大学齐鲁医学院,山东 济南 250012
- 2. 山东大学齐鲁医学院,山东 济南 250012;济宁医学院附属医院病理科,山东 济宁 272029
- 折叠
摘要
目的 探讨结直肠癌(colorectal cancer,CRC)中含溴结构域蛋白 9(bromodomain containing 9,BRD9)的蛋白表达水平、亚细胞定位模式及其临床意义.方法 收集CRC手术切除标本,采用免疫组织化学技术EnVision法检测BRD9 的蛋白表达水平及其亚细胞定位,并分析其与患者临床病理特征及预后的关系.结果 在 508 例CRC组织标本中,BRD9 高表达患者为 71.46%(363/508),BRD9 细胞核阳性患者为 80.31%(408/508);BRD9 的表达与患者肿瘤大小(P=0.025)、淋巴结转移(P=0.004)、神经侵犯(P=0.043)、分化程度(P=0.017)及TNM分期(P=0.002)相关,BRD9 的亚细胞定位与患者淋巴结转移(P=0.040)及TNM分期(P=0.026)相关,差异有统计学意义;BRD9 高表达可作为患者的独立不良预后因素(HR=1.994,P=0.002),同时,在 BRD9 低表达患者中,BRD9 细胞浆阳性可作为患者的独立不良预后因素(HR=8.056,P=0.001).结论 在CRC患者中BRD9 的亚细胞定位模式具有异质性,其高表达及细胞浆定位与CRC的发生发展密切相关,并能够作为独立预测因子预测患者的生存预后.
Abstract
Objective To investigate the protein expression level,subcellular localization pattern and clinical signifi-cance of bromodomain containing 9(BRD9)in colorectal cancer(CRC).Methods Surgical resection specimens of CRC were collected,and immunohistochemistry(EnVision method)was used to detect BRD9 protein expression and subcellular localization.The relationship between BRD9 expression and clinicopathological features and prognosis of CRC patients was analyzed.Results Among 508 CRC tissue specimens,BRD9 was overexpressed in 71.46%(363/508)of patients,and 80.31%(408/508)of patients showed nuclear positive staining in immunohistochemistry for BRD9.The expression of BRD9 was correlated with tumor size(P=0.025),lymph node metastasis(P=0.004),nerve invasion(P=0.043),differentiation grade(P=0.017)and TNM stage(P=0.002).The subcellular localization of BRD9 was correlated with lymph node metastasis(P=0.040)and TNM stage(P=0.026).The difference was statisti-cally significant.Meanwhile,BRD9 high expression served as an independent poor prognostic factor(HR=1.994,P=0.002).More important,in patients with BRD9 low expression,cytoplasmic BRD9 expression was an independent poor prognostic factor of patients(HR=8.056,P=0.001).Conclusion The subcellular localization of BRD9 is heterogene-ous in CRC patients.The high expression and cytoplasmic localization of BRD9 are related to the occurrence and devel-opment of CRC,and could be used as an independent predictor for the prognosis of CRC patients.
关键词
结直肠癌/免疫组织化学/含溴结构域蛋白9/亚细胞定位/预后Key words
Colorectal cancer/Immunohistochemistry/Bromodomain containing 9/Subcellular localization/Prognosis引用本文复制引用
基金项目
国家自然科学基金(81902815)
国家自然科学基金(81802786)
山东省自然科学基金(ZR2023MH011)
山东省自然科学基金(ZR2019BH044)
山东省自然科学基金(ZR2018BH025)
出版年
2024
NETL
NSTL
万方数据