α5-nAChR与MHC-Ⅰ在肺腺癌中的表达及相关性
Expression and correlation of α5-nAChR and MHC-Ⅰ in lung adenocarcinoma
王靖婷 1王璟 1鲁艺 1李静坦 1李强 1郏雁飞 1马晓丽1
作者信息
- 1. 山东第一医科大学附属中心医院基础医学研究中心,山东济南 250013
- 折叠
摘要
目的 探讨α5-烟碱型乙醜胆碱受体(alpha5-nicotinic acetylcholine receptor,α5-nAChR)与主要组织相容性复合体Ⅰ类分子(major histocompatibility complex class Ⅰ molecule,MHC-Ⅰ)在肺腺癌中的表达及相关性.方法 TC-GA数据库分析CHRNA5(编码α5-nAChR基因)与HLA-B(编码MHC-Ⅰ基因)在肺腺癌中的表达、相关性及其临床意义.运用免疫组织化学技术检测人肺腺癌临床样本、裸鼠肺腺癌异种移植瘤组织中α5-nAChR与MHC-Ⅰ的表达及相关性.在人A549细胞、小鼠LLC细胞中,Western blotting检测在α5-nAChR不同表达水平下的FHIT与MHC-Ⅰ表达及相关性.结果 数据库分析表明,CHRNA5高表达或HLA-B低表达肺腺癌患者的生存率降低,CHRNA5与HLA-B的表达呈负相关(P＜0.05).α5-nAChR与MHC-Ⅰ在人肺腺癌、裸鼠肺腺癌异种移植物中的表达呈负相关(P＜0.05).在肺腺癌细胞中,α5-nAChR的表达分别与FHIT、MHC-Ⅰ的表达呈负相关(P＜0.05),FHIT和MHC-Ⅰ在肺腺癌细胞中的表达呈正相关(P＜0.05).结论 α5-nAChR与MHC-Ⅰ的表达呈负相关,并参与肺腺癌的发生.
Abstract
Objective To investigate the expression and correlation between alpha5-nicotinic acetylcholine receptor(α5-nAChR)and the major histocompatibility complex class Ⅰ molecule(MHC-Ⅰ)in lung adenocarcinoma.Methods TCGA database were used to study the expression,correlation,and clinical significance of encoding α5-nAChR gene(CHRNA5)and encoding MHC-Ⅰ gene(HLA-B)in lung adenocarcinoma.The expressions of α5-nAChR and MHC-Ⅰwere tested by immunohistochemistry staining in human lung adenocarcinoma specimans and nude mouse lung adenocar-cinoma xenografts tissues.Furthermore,the expression and correlation of FHIT and MHC-Ⅰ at different expression levels of α5-nAChR were detected by Western blotting in human A549 cells and mice LLC cells.Results Patients with lung adenocarcinoma with high expression of CHRNA5 or low expression of HLA-B had reduced survival,and CHRNA5 was negatively correlated with the expression of HLA-B(P＜0.05).The expressions of α5-nAChR and MHC-Ⅰ were negatively correlated in human lung adenocarcinoma and nude mouse lung adenocarcinoma xenografts tissues(P＜0.05).In lung adenocarcinoma cells,the expression of α5-nAChR was negatively correlated with the expressions of FHIT and MHC-Ⅰ,and the expression of FHIT and MHC-Ⅰ was positively correlated(P＜0.05).Conclusion The expressions ofα5-nAChR and MHC-Ⅰ are negatively correlated and involved in lung adenocarcinogenesis.
关键词
α5-烟碱型乙酰胆碱受体/脆性组氨酸三联体/主要组织相容性复合体Ⅰ类分子/肺腺癌/相关性Key words
Alpha5-nicotinic acetylcholine receptor/Fragile histidine triad/Major histocompatibility complex class Ⅰmolecule/Lung adenocarcinoma/Correlation引用本文复制引用
基金项目
山东省自然科学基金面上项目(ZR2021MH322)
出版年
2024
NETL
NSTL
万方数据