Expression and diagnostic value of plasma exosomal miR-548k in esophageal squamous cell carcinoma
Objective To screen out the candidate plasma exosomal microRNAs(miRNAs)which play crucial role in esophageal squamous cell carcinoma(ESCC)occurrence and evaluate their potential in ESCC diagnosis.Methods A total of 55 ESCC patients who underwent surgical resection at The Second Hospital of Shandong University from March 2020 to June 2021 and 55 healthy volunteers were enrolled in this study.After identified by transmission electron micros-copy(TEM),NanoSight tracking analysis(NTA)and Western blotting assays,RNA sequencing was performed to de-termine the miRNA profiles of plasma exosomes from 5 ESCC patients and 5 healthy control.Then,quantitative real-time PCR(qRT-PCR)was used to detect the expression level of differential exosomal miRNAs in the training and vali-dation sets.Then,receiver operating characteristic(ROC)curve was conducted to evaluate their diagnostic value.Results Five miRNAs(miR-548k,miR-6516-5p,let-7b-3p,miR-3934-5p,miR-196a-5p)were significantly higher,and 8 miRNAs(miR-34c-5p,novel_167,miR-548u,miR-548ah-3p,miR-548p,miR-200a-5p,miR-200b-3p,miR-1228-5p)were obviously decreased in plasma exosomes of ESCC group than that of control group.Additionally,through the training cohort,we verified that miR-548k was significantly up-regulated in plasma exosomes of ESCC group(P＜0.001).ROC curve analysis revealed that exosomal miR-548k exhibited higher accuracy than miR-34c-5p,miR-548u,miR-6516-5p in ESCC diagnosis,and the area under ROC curve(AUC)value was 0.918.Finally,through the validation cohort,we further confirmed that miR-548k was highly expressed in plasma exosomes of ESCC group(P＜0.001),and plasma exosomal miR-548k could be used for ESCC diagnosis(AUC=0.889).Conclusion MiR-548k is significantly up-regulated in plasma exosomes of ESCC patients and plasma exosomal miR-548k has high clini-cal diagnostic value for ESCC.
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