Intratumor heterogeneity of SP1 expression in colorectal cancer and its clinical significance
Objective To investigate the intratumor heterogeneity(ITH)of specificity protein 1(SP1)protein expres-sion in colorectal cancer(CRC)and its clinical significance.Methods Clinical specimens of CRC were collected.The expression level and pattern of SP1 protein were detected by immunohistochemical EnVision method.The ITH character-istics of SP1 protein expression were identified,and the ITH level of SP1 protein was defined.The relationship between ITH level of SP1 protein and clinicopathological characteristics and prognosis of CRC patients was analyzed.Results SP1 protein showed typical ITH expression in CRC.The ITH level of SP1 protein was associated with lymph node me-tastasis(P＜0.001)and TNM stage(P＜0.001).Survival analysis showed that the overall survival(OS)of patients with high ITH level of SP1 protein was significantly lower than that with low ITH level(61.25％vs 81.82％,P=0.004).Univariate and multivariate Cox regression analyses showed that high SP1 protein ITH was an independent pre-dictor of poor OS in CRC patients(HR=2.680,P=0.017).Conclusion The expression of SP1 protein in CRC has significant ITH characteristics,and defines the quantitative criteria of SP1 ITH level for the first time.It is demonstrated that a high ITH level of SP1 protein is significantly associated with CRC progression and poor prognosis.
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