Role of microfibril-associated protein 3 in regulating mesenchymal transition of glioma stem cells
Objective To investigate the expression and clinical significance of microfibril-associated protein 3(MFAP3)in glioblastoma(GBM)and its influence on the malignant biological behavior of glioma stem cells(GSCs).Methods TCGA data and CGGA data were used to analyze the expression of MFAP3 in GBM and its correlation with patient prognosis.Western blotting was used to detect the protein expression level of MFAP3 in GSCs.The effects of MFAP3 knockdown and overexpression on the self-renewal ability and phenotype of GSCs were explored.Results MFAP3 was highly expressed in GBM and correlated with poor prognosis.The expression level of MFAP3 in mesenchymal GSCs was higher than that in preneuronal GSCs.After MFAP3 knockdown,the self-renewal ability of GSCs was weakened,and the protein levels of mesenchymal phenotype markers CD44 and YKL40 were decreased,whereas overexpression exhibited the opposite trends.Conclusion MFAP3 plays an important role in regulating the ma-lignant biological progression of GBM and is expected to be a new clinical diagnostic marker and therapeutic intervention target for GBM.
Microfibril-associated protein 3Glioma stem cellsPhenotype transitionSelf-renewalPrognosis
NETL
NSTL
万方数据
