Relationship between fusobacterium nucleatum mediated autophagy and cell refractive index based on optical surface wave
Objective To explore the relationship between autophagy levels mediated by Fusobacterium nucleatum(Fn)and changes in cellular refractive index using optical surface wave sensing technology and to preliminarily investi-gates the potential of optical surface wave technology for detecting autophagy levels in cancer cells.Methods CCK-8 was used to assess the impact of Fn on the proliferation activity of colorectal cancer cells.The effects of Fn on autoph-agy levels were examined using both Western blotting and dual-labeling fluorescence microscopy techniques.Additional-ly,optical surface wave technology was employed to monitor changes in cellular refractive index under different Fn infection states,and to observe their correlation with autophagy levels.Results When multiplicity of infection was 100 and the infection time was 4 hours,the protein expression levels of Beclin-1 and LC3 were increased(P<0.05),while the expression level of P62 was decreased(P<0.01),indicating elevated autophagy levels.Concurrently,optical sur-face wave showed that the refractive indices of HCT116 and HT29 cells also increased with the elevation of autophagy levels(P<0.01).Further investigation revealed that changes in cellular refractive index were primarily due to variations in autophagosome levels within the cells,and were not influenced by factors such as cell proliferation or extracellular substances.Conclusion The autophagy levels mediated by Fn correlate with cellular refractive index,indicating that optical surface wave sensing technology can dynamically reflect changes in Fn-mediated autophagy levels.This suggests that optical surface wave technology could serve as a rapid,intuitive,and non-invasive method to assess autophagy lev-els without affecting cellular viability.
Fusobacterium nucleatumColorectal cancerAutophagyOptical surface wave techniqueCell refractive index
万方数据
维普
